Proteomics

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O-GalNAc–glycosylated nuclear proteins


ABSTRACT: Biological functions of nuclear proteins are regulated by post-translational modifications (PTMs) that modulate gene expression and cellular physiology. However, the role of O-linked glycosylation (O-GalNAc) as a PTM of nuclear proteins in the human cell has not been previously reported. Here, we examined the initiation of O-GalNAc glycan biosynthesis, representing a novel PTM of nuclear proteins in the nucleus of human cells, with an emphasis on HeLa cells. Using affinity chromatography and MS analyses, we identified O-GalNAc glycosylated proteins in the nucleus and present solid evidence for O-GalNAc glycan synthesis in this organelle. The demonstration of O-GalNAc glycosylation of nuclear proteins in mammalian cells reported here has important implications for cell and chemical biology.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Cervix Carcinoma

SUBMITTER: ROMINA CEJAS  

LAB HEAD: FERNANDO J IRAZOQUI

PROVIDER: PXD013484 | Pride | 2019-04-17

REPOSITORIES: Pride

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Publications

Biosynthesis of <i>O-N</i>-acetylgalactosamine glycans in the human cell nucleus.

Cejas Romina B RB   Lorenz Virginia V   Garay Yohana C YC   Irazoqui Fernando J FJ  

The Journal of biological chemistry 20181227 9


Biological functions of nuclear proteins are regulated by post-translational modifications (PTMs) that modulate gene expression and cellular physiology. However, the role of <i>O-</i>linked glycosylation (<i>O</i>-GalNAc) as a PTM of nuclear proteins in the human cell has not been previously reported. Here, we examined in detail the initiation of <i>O-</i>GalNAc glycan biosynthesis, representing a novel PTM of nuclear proteins in the nucleus of human cells, with an emphasis on HeLa cells. Using  ...[more]

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