De novo Classification of Mouse B Cell Types using Surfaceome Proteotype Maps

ABSTRACT: System-wide quantification of the cell surface proteotype and identification of extracellular glycosylation sites is challenging when sample is limiting. We miniaturized and automated the previously described Cell Surface Capture technology increasing sensitivity, reproducibility, and throughput. We used this technology, which we call autoCSC, to create population-specific surfaceome maps of developing mouse B cells and used targeted flow cytometry to uncover developmental cell subpopulations.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Primary Cell, Cell Culture, Bone Marrow, Blood, Hela Cell

SUBMITTER: Maik Müller  

LAB HEAD: Bernd Wollscheid

PROVIDER: PXD013627 | Pride | 2019-12-11

REPOSITORIES: Pride