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Bovine Coronary Artery Endothelial Cells-SWATH Proteomics

ABSTRACT: Here, we evaluated the appearance and abundance of Multinucleated varian endothelial cells after prolonged exposure (9 days) to high-glucose and high-insulin and characterized their morphology, as well as their mitochondrial mass and function. In addition, we performed a quantitative proteomic differential analysis among endothelial cells cultured under normal-glucose and high glucose+high-insulin.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Cell Culture, Endothelial Cell Of Coronary Artery

DISEASE(S): Type 2 Diabetes Mellitus

SUBMITTER: Aldo Moreno

LAB HEAD: Aldo Moreno Ulloa

PROVIDER: PXD013643 | Pride | 2022-08-11

REPOSITORIES: Pride

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Publications

Multi-omics study identifies novel signatures of DNA/RNA, amino acid, peptide, and lipid metabolism by simulated diabetes on coronary endothelial cells.

Moreno-Ulloa Aldo A Delgado-De la Herrán Hilda Carolina HC Álvarez-Delgado Carolina C Mendoza-Porras Omar O Carballo-Castañeda Rommel A RA Donis-Maturano Luis L Villarreal Francisco F

Scientific reports 20220714 1

Coronary artery endothelial cells (CAEC) exert an important role in the development of cardiovascular disease. Dysfunction of CAEC is associated with cardiovascular disease in subjects with type 2 diabetes mellitus (T2DM). However, comprehensive studies of the effects that a diabetic environment exerts on this cellular type are scarce. The present study characterized the molecular perturbations occurring on cultured bovine CAEC subjected to a prolonged diabetic environment (high glucose and high ...[more]

PMID: 35835939

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