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The equine granulocyte proteome: expression changes in autoimmune disease and after stimulation

ABSTRACT: We investigated the granulocyte proteome from healthy horses, horses with a spontaneous autoimmune disease (equine recurrent uveitis) and the reaction of granulocytes to different stimuli (IL8, PMA, LPS) on protein level. A total of 2032 proteins describing the whole granulocyte proteome were identified, including 245 proteins (12% of proteome) newly associated to in vivo expression in primary granulocytes (hypothetical proteins). Our studies provide novel information on the granulocyte proteome and contribute to a better understanding of molecular mechanisms involved in activation and recruitment of neutrophils and the role of these cells in modulating and regulating autoimmune diseases. The data presented here is highly relevant for veterinary medicine and has translational quality for spontaneous autoimmune uveitis in man.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Equus Caballus (horse)

TISSUE(S): Primary Cell, Leukocyte, Neutrophil, Blood

SUBMITTER: Roxane Degroote

LAB HEAD: Cornelia A Deeg

PROVIDER: PXD013648 | Pride | 2020-01-15

REPOSITORIES: Pride

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IL8 and PMA Trigger the Regulation of Different Biological Processes in Granulocyte Activation.

Degroote Roxane L RL Weigand Maria M Hauck Stefanie M SM Deeg Cornelia A CA

Frontiers in immunology 20200114

The molecular mechanisms driving specific regulation of neutrophils are not completely understood to date. In order to characterize fundamental granulocyte features on protein level, we analyzed changes in proteome composition as reaction to stress from cell activation processes. For this purpose, we isolated primary granulocytes from equine whole blood through density gradient centrifugation followed by sodium chloride lysis and stimulated cells for 30 min with interleukin-8 (IL8) due to its ro ...[more]

PMID: 32010136

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