Dataset Information

NanoLC-MSMS Phosphoproteomics comparison of in vitro cultured reticulocytes before and after passage through microfluidic capillaries

ABSTRACT: Triplicate samples (biological replicates) of in vitro culture-derived reticulocytes (derived from CD34+ cells) were analysed through qualitative phosphoproteomics before and after passage through a microfluidics capillary chip to investigate the mechanisms underlying shear stress recognition.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Suspension Culture, Cell Culture, Reticulocyte, Blood

SUBMITTER: Pedro Moura

LAB HEAD: Ashley Mark Toye

PROVIDER: PXD013652 | Pride | 2019-09-12

REPOSITORIES: Pride

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Publications

Reticulocyte and red blood cell deformation triggers specific phosphorylation events.

Moura Pedro L PL Lizarralde Iragorri Maria A MA Français Olivier O Le Pioufle Bruno B Dobbe Johannes G G JGG Streekstra Geert J GJ El Nemer Wassim W Toye Ashley M AM Satchwell Timothy J TJ

Blood advances 20190901 17

The capacity to undergo substantial deformation is a defining characteristic of the red blood cell (RBC), facilitating transit through the splenic interendothelial slits and microvasculature. Establishment of this remarkable property occurs during a process of reticulocyte maturation that begins with egress through micron-wide pores in the bone marrow and is completed within the circulation. The requirement to undertake repeated cycles of deformation necessitates that both reticulocytes and eryt ...[more]

PMID: 31506283

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Dataset Information

NanoLC-MSMS Phosphoproteomics comparison of in vitro cultured reticulocytes before and after passage through microfluidic capillaries

Publications

Reticulocyte and red blood cell deformation triggers specific phosphorylation events.

Similar Datasets

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