MS analysis of purified TRIM46, 480AnkG and 480AnkG-NN.
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Cell Culture
SUBMITTER: 
Riccardo Stucchi
LAB HEAD: Casper Hoogenraad
PROVIDER: PXD013685 | Pride | 2019-08-30
REPOSITORIES: Pride
Publications
Feedback-Driven Assembly of the Axon Initial Segment.
Fréal Amélie A Rai Dipti D Tas Roderick P RP Pan Xingxiu X Katrukha Eugene A EA van de Willige Dieudonnée D Stucchi Riccardo R Aher Amol A Yang Chao C Altelaar A F Maarten AFM Vocking Karin K Post Jan Andries JA Harterink Martin M Kapitein Lukas C LC Akhmanova Anna A Hoogenraad Casper C CC
Neuron 20190829 2
The axon initial segment (AIS) is a unique neuronal compartment that plays a crucial role in the generation of action potential and neuronal polarity. The assembly of the AIS requires membrane, scaffolding, and cytoskeletal proteins, including Ankyrin-G and TRIM46. How these components cooperate in AIS formation is currently poorly understood. Here, we show that Ankyrin-G acts as a scaffold interacting with End-Binding (EB) proteins and membrane proteins such as Neurofascin-186 to recruit TRIM46 ...[more]
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