Extracellular vesicles impose quiescence and confer DNA-damage on residual HSC in the AML niche

ABSTRACT: To understand the mechanism by which AML-EVs may enforce quiescence, we performed tandem mass tag (TMT) proteomic profiling of in vitro cultured c-Kit+ hematopoietic stem and progenitor cells (HSPC) (to obtain the minimum required amount of protein lysates) treated with extracellular vesicles (EVs) for 48 hours from human HL-60 or Molm-14 cell cultures versus controls.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Hematopoietic Stem Cell, Bone Marrow

DISEASE(S): Acute Leukemia

SUBMITTER: Phillip Wilmarth  

LAB HEAD: Dr. Peter Kurre

PROVIDER: PXD013688 | Pride | 2019-06-24

REPOSITORIES: Pride