Dataset Information

Energetics- and transport-related protein abundance responds differentially to salt in Oryza australiensis roots

ABSTRACT: Salinity is a major constraint on agricultural productivity worldwide. Despite the serious impacts of salinity on rice yields, particularly in Asia, mechanisms of salt tolerance in wild rice species are yet to be explored. Here we extracted and quantified root microsomal proteins of Oryza australiensis accessions contrasting in salt tolerance. Whole root systems of two-week-old seedlings were treated with 80 mM NaCl for 30 days or left untreated. Proteins were quantified by tandem mass tags (TMT) and triple-stage MS. We found >200 differentially expressed proteins (DEPs) between the salt-treated and control in the two accessions (p-value <0.05). Gene Ontology (GO) analysis showed that ‘metabolic process’, ‘transport’ and ‘transmembrane transporter’ activities were highly responsive categories following salt treatment of the O. australiensis seedlings. In particular, ATPases and SNARE proteins were up-regulated in the salt-tolerant accession and appeared to have a major role in response to salinity. ATPases are the central link between energy generation and transport, while SNARE proteins facilitate vesicle fusion and interact with voltage-gated potassium channels to regulate K+ influx. We successfully validated the putative function of two strongly upregulated proteins, a monosaccharide transporter and a VAMP-like protein, by measuring the growth under salinity of yeast mutants in which homologous genes were deleted. Our results demonstrate the potential use of wild species as a source of new mechanisms of salt tolerance for the breeding of elite cultivars of rice.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Oryza Australiensis

TISSUE(S): Plant Cell, Root

SUBMITTER: Yoav Yichie

LAB HEAD: Thomas H Roberts

PROVIDER: PXD013701 | Pride | 2019-09-11

REPOSITORIES: Pride

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Publications

Salinity tolerance in Australian wild Oryza species varies widely and matches that observed in O. sativa.

Yichie Yoav Y Brien Chris C Berger Bettina B Roberts Thomas H TH Atwell Brian J BJ

Rice (New York, N.Y.) 20181222 1

<h4>Background</h4>Soil salinity is widespread in rice-producing areas globally, restricting both vegetative growth and grain yield. Attempts to improve the salt tolerance of Asian rice, Oryza sativa-the most salt sensitive of the major cereal crops-have met with limited success, due to the complexity of the trait and finite variation in salt responses among O. sativa lines. Naturally occurring variation among the more than 20 wild species of the Oryza genus has great potential to provide breede ...[more]

PMID: 30578452

Publication: 1/2

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Dataset Information

Energetics- and transport-related protein abundance responds differentially to salt in Oryza australiensis roots

Publications

Salinity tolerance in Australian wild Oryza species varies widely and matches that observed in O. sativa.

Similar Datasets

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