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Mechanism of antimicrobial synergy between doxycycline and carprofen against methicillin-resistant Staphylococus pseudintermedius

ABSTRACT: We previously showed that doxycycline and carprofen , a veterinary non-steroidal anti-inflammatory drug, have synergistic antimicrobial activity against methicillin-resistant Staphylococus pseudintermedius (MRSP) carrying the tetracycline resistance determinant TetK. To elucidate the molecular mechanism of this synergy, we investigated the effects of the two drugs, individually and in combination, using a comprehensive approach including two-dimensional differential in-gel electrophoresis (2D DIGE).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Staphylococcus Pseudintermedius

SUBMITTER: Zofia Magnowska

LAB HEAD: Luca Guardabassi

PROVIDER: PXD013725 | Pride | 2020-05-26

REPOSITORIES: Pride

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Carprofen-induced depletion of proton motive force reverses TetK-mediated doxycycline resistance in methicillin-resistant Staphylococcus pseudintermedius.

Magnowska Zofia Z Jana Bimal B Brochmann Rikke Prejh RP Hesketh Andrew A Lametsch Rene R De Gobba Cristian C Guardabassi Luca L

Scientific reports 20191128 1

We previously showed that doxycycline (DOX) and carprofen (CPF), a veterinary non-steroidal anti-inflammatory drug, have synergistic antimicrobial activity against methicillin-resistant Staphylococcus pseudintermedius (MRSP) carrying the tetracycline resistance determinant TetK. To elucidate the molecular mechanism of this synergy, we investigated the effects of the two drugs, individually and in combination, using a comprehensive approach including RNA sequencing, two-dimensional differential i ...[more]

PMID: 31780689

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