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Sparrow seminal fluid diversification -MS/MS

ABSTRACT: We investigated seminal fluid (SF) diversification in a recently diverged passerine species pair (Passer domesticus and P. hispaniolensis) using a combination of proteomic and comparative evolutionary genomic approaches. Specifically, using tandem MS/MS semi-quantitative proteomic methods we identified and compared the SF proteome of two species of Passer sparrows - the house and Spanish sparrow. This analysis revealed consistencies with known aspects of SF protein biology and function in other taxa, including the presence of a diversity of immune and antimicrobial proteins

INSTRUMENT(S): Q Exactive

ORGANISM(S): Passer Hispaniolensis Passer Domesticus Domesticus

TISSUE(S): Seminal Plasma

SUBMITTER: Melissah Rowe

LAB HEAD: Melissah Rowe

PROVIDER: PXD013748 | Pride | 2019-11-06

REPOSITORIES: Pride

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Molecular Diversification of the Seminal Fluid Proteome in a Recently Diverged Passerine Species Pair.

Rowe Melissah M Whittington Emma E Borziak Kirill K Ravinet Mark M Eroukhmanoff Fabrice F Sætre Glenn-Peter GP Dorus Steve S

Molecular biology and evolution 20200201 2

Seminal fluid proteins (SFPs) mediate an array of postmating reproductive processes that influence fertilization and fertility. As such, it is widely held that SFPs may contribute to postmating, prezygotic reproductive barriers between closely related taxa. We investigated seminal fluid (SF) diversification in a recently diverged passerine species pair (Passer domesticus and Passer hispaniolensis) using a combination of proteomic and comparative evolutionary genomic approaches. First, we charact ...[more]

PMID: 31665510

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