E. coli Affinity purification MS/MS

ABSTRACT: The tRNA linked repeat sequence rrB was tagged in either 5'- or 3'- end with the MS2 aptamer and expressed from a plasmid in E. coli. Using the MS2 aptamer, the RNAs were affinity purified after cell lysis. The co-purifying proteins were identified by NanoLC-MS/MS and compared to the proteins co-purifying with the MS2 sequence alone.

INSTRUMENT(S):

ORGANISM(S): Escherichia Coli

TISSUE(S): Cell Culture

SUBMITTER: Thoams Stenum  

LAB HEAD: Michael Askvad Sørensen

PROVIDER: PXD013749 | Pride | 2021-04-26

REPOSITORIES: Pride