Proteomics

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Identification of MDN1-dependent proteome changes in Arabidopsis seeds

ABSTRACT: mdn1-1 is a weak mutant of the Arabidopsis MDN1 gene. mdn1-1 exhibits an increased seed-size phenotype. To further figure out the proteome changes in the mdn1-1 seeds, we applied the iTRAQ-based LC-MS/MS analysis. A total of 3217 proteins were identified in dry seeds of both wild type and mdn1-1. The significantly differentially expressed proteins (DEPs) were filtered out based on the fold change (FC > 1.5 or < 0.667) of expression level and P value (< 0.05). We defined 282 up- and 40 downregulated proteins in mdn1-1.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Seed

SUBMITTER: Pengcheng Li  

LAB HEAD: Li pengcheng

PROVIDER: PXD013784 | Pride | 2019-11-12

REPOSITORIES: Pride

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Publications

<i>Arabidopsis</i> MDN1 Is Involved in the Establishment of a Normal Seed Proteome and Seed Germination.

Li Peng-Cheng PC   Ma Jun-Jie JJ   Zhou Xi-Meng XM   Li Guang-Hui GH   Zhao Chuan-Zhi CZ   Xia Han H   Fan Shou-Jin SJ   Wang Xing-Jun XJ  

Frontiers in plant science 20190910

Seed germination and formation are the beginning and ending, respectively, of a plant life cycle. These two processes are under fine regulation by the internal genetic information. Previously, we demonstrated that <i>Arabidopsis</i> MIDASIN 1 (MDN1) is required for ribosome biogenesis, and its dysfunction leads to pleiotropic developmental phenotypes, including impaired embryogenesis and slow seed germination. In this study, we further found that the weak mutant of <i>MDN1</i>, <i>mdn1-1</i>, ex  ...[more]

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