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A screen to indentify non-covalent isotype-specific SUMO binders

ABSTRACT: In this study we identify SUMO isotype-specific non covalent binders. The different isotypes include SUMO1, SUMO2 and a 3xSUMO2 chain.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Román González-Prieto

LAB HEAD: Alfred C.O. Vertegaal

PROVIDER: PXD013842 | Pride | 2021-01-29

REPOSITORIES: Pride

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Global non-covalent SUMO interaction networks reveal SUMO-dependent stabilization of the non-homologous end joining complex.

González-Prieto Román R Eifler-Olivi Karolin K Claessens Laura A LA Willemstein Edwin E Xiao Zhenyu Z Talavera Ormeno Cami M P CMP Ovaa Huib H Ulrich Helle D HD Vertegaal Alfred C O ACO

Cell reports 20210101 4

In contrast to our extensive knowledge on covalent small ubiquitin-like modifier (SUMO) target proteins, we are limited in our understanding of non-covalent SUMO-binding proteins. We identify interactors of different SUMO isoforms-monomeric SUMO1, monomeric SUMO2, or linear trimeric SUMO2 chains-using a mass spectrometry-based proteomics approach. We identify 379 proteins that bind to different SUMO isoforms, mainly in a preferential manner. Interestingly, XRCC4 is the only DNA repair protein in ...[more]

PMID: 33503430

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