Proteomics

Dataset Information

4

Alterations in lipid profile of the aging kidney identified by MALDI imaging mass spectrometry


ABSTRACT: During aging, the kidney undergoes functional and physiological changes that are closely affiliated with chronic kidney disease (CKD). There is increasing evidence supporting the role of lipid or lipid-derived mediators in the pathogenesis of CKD and other aging-related diseases. To understand the role of lipids in various metabolic processes during kidney aging, we conducted MALDI imaging mass spectrometry (MALDI-IMS) analysis in kidneys harvested from young (2 months old, n=3) and old mice (24 months old, n=3). MALDI-IMS analysis showed an increase in ceramide level and a decrease in sphingomyelin (SM) and phosphatidylcholine (PC) levels in kidneys of old mice. The increased expression of cPLA2 and SMPD1 protein in aged kidney was confirmed by immunohistochemistry and western blot analysis. Our MALDI-IMS data showed the altered distribution of lipids in aged kidney as indicative of aging-related functional changes of the kidney. Combined analysis of MALDI-IMS and IHC confirmed lipidomic changes and expression levels of responsible enzymes as well as morphological changes.

INSTRUMENT(S): Bruker Daltonics flex series

ORGANISM(S): Mus musculus  

TISSUE(S): Kidney

DISEASE(S): Disease Free,Chronic Kidney Disease

SUBMITTER: Joon Won Lee  

LAB HEAD: Kwang Pyo Kim

PROVIDER: PXD013860 | Pride | 2019-06-10

REPOSITORIES: pride

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Alterations in Lipid Profile of the Aging Kidney Identified by MALDI Imaging Mass Spectrometry.

Noh Sue Ah SA   Kim Su-Mi SM   Park Seon Hwa SH   Kim Dong-Jin DJ   Lee Joon Won JW   Kim Yang Gyun YG   Moon Ju-Young JY   Lim Sung-Jig SJ   Lee Sang-Ho SH   Kim Kwang Pyo KP  

Journal of proteome research 20190619 7


During aging, the kidney undergoes functional and physiological changes that are closely affiliated with chronic kidney disease (CKD). There is increasing evidence supporting the role of lipid or lipid-derived mediators in the pathogenesis of CKD and other aging-related diseases. To understand the role of lipids in various metabolic processes during kidney aging, we conducted matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS) analysis in kidneys harvested from youn  ...[more]

Publication: 1/2

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