Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Trypanosoma cruzi PAN 4 exosomes LC-MS/MS

ABSTRACT: Here we report the proteome content of exosomes released by epimastigote and bloodstream trypomastigotes forms of T. cruzi, the aetiological agent of Chagas disease. The exosomes were isolated using the ultracentrifugation method and further processed for LC-MS/MS. The results showed differential proteomic profiles of this two forms with marked differences in surface molecules cargoes. Noteworthy, the trans-sialidase (TS) surface proteins, a family of polymorphic virulence factors were differentially expressed between insect and human stages of T. cruzi highlighting the different surface nature of this two parasite stages.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Trypanosoma Cruzi Strain Cl Brener

DISEASE(S): Chagas Disease

SUBMITTER: Luis Miguel de Pablos Torró

LAB HEAD: Dr. Antonio Osuna Carrillo de Albornoz

PROVIDER: PXD013878 | Pride | 2021-09-08

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Publications

Biophysical and Biochemical Comparison of Extracellular Vesicles Produced by Infective and Non-Infective Stages of <i>Trypanosoma cruzi</i>.

Retana Moreira Lissette L Prescilla-Ledezma Alexa A Cornet-Gomez Alberto A Linares Fátima F Jódar-Reyes Ana Belén AB Fernandez Jorge J Ibarrola Vannucci Ana Karina AK De Pablos Luis Miguel LM Osuna Antonio A

International journal of molecular sciences 20210513 10

Extracellular vesicles (EVs) are small lipid vesicles released by either any prokaryotic or eukaryotic cell, or both, with a biological role in cell-to-cell communication. In this work, we characterize the proteomes and nanomechanical properties of EVs released by tissue-culture cell-derived trypomastigotes (mammalian infective stage; (TCT)) and epimastigotes (insect stage; (E)) of <i>Trypanosoma cruzi</i>, the etiologic agent of Chagas disease. EVs of each stage were isolated by differential ce ...[more]

PMID: 34068436

Similar Datasets

Trypanosoma cruzi PAN 4 exosomes LC-MS/MS

Project description:Here we report the proteome content of exosomes released by epimastigote and bloodstream trypomastigotes forms of T. cruzi, the aetiological agent of Chagas disease. The exosomes were isolated using the ultracentrifugation method and further processed for LC-MS/MS. The results showed differential proteomic profiles of this two forms with marked differences in surface molecules cargoes. Noteworthy, the trans-sialidase (TS) surface proteins, a family of polymorphic virulence factors were differentially expressed between insect and human stages of T. cruzi highlighting the different surface nature of this two parasite stages.

2021-09-08 | PXD013901 | Pride

Trypanosoma cruzi PAN 4 exosomes LC-MS/MS

2021-09-08 | PXD013882 | Pride

Trypanosoma cruzi PAN 4 exosomes LC-MS/MS

2021-09-08 | PXD013876 | Pride

Trypanosoma cruzi PAN 4 exosomes LC-MS/MS

2021-09-08 | PXD013875 | Pride

Trypanosoma cruzi PAN 4 exosomes LC-MS/MS

2021-09-08 | PXD013880 | Pride

Stable Isotope Labeling using Amino acids in Cell culture (SILAC) (mus.musculus)

Project description:Targeted SILAC determination of the rate of synthesis of proteins involved in mitochondrial structure and ETC activity in CT26 control and IF1-ablated cell lines

2023-10-24 | PXD038108 | Pride

Stable Isotope Labeling using Amino acids in Cell culture (SILAC) (local data base)

Project description:Stable Isotope Labeling using Amino acids in Cell culture (SILAC) of CT26 control and IF1-ablated cells

2023-10-24 | PXD038109 | Pride

Identification of muscle biomarkers in McArdle disease

Project description:Glycogen storage disease type V (GSDV, McArdle disease) is a rare genetic myopathy caused by deficiency of the muscle isoform of glycogen phosphorylase (PYGM). This results in a block in the use of muscle glycogen as an energetic substrate, with subsequent exercise intolerance. The GSDV pathophysiology is still not fully understood, especially with regard to some features such as muscle contractures or persistent muscle damage (i.e., even without prior exercise). We aimed at identifying muscle protein biomarkers of GSDV by analyzing the muscle proteome and the molecular networks associated with muscle dysfunction. Muscle biopsies from 8 patients and 8 controls were studied by quantitative protein expression using isobaric tags for relative and absolute quantitation (iTRAQ) followed by artificial neuronal networks (ANNs) and topology analysis. Protein candidate validation was performed by western-blot. Several proteins predominantly involved in the process of muscle contraction and/or calcium homeostasis, such as myosin, sarcoplasmic/endoplasmic reticulum calcium ATPase 1, tropomyosin alpha-1 chain, tro-ponin isoforms, and alpha-actinin-3 showed significantly lower expression levels in muscle of GSDV patients. These proteins could be potential biomarkers of the persistent muscle damage reported in GSDV patients. Further studies are needed to elucidate the molecular mechanisms by which PYGM controls the expression of these proteins.

2022-06-09 | PXD031605 | Pride

Cyclipostins and Cyclophostin analogs impair growth of Mycobacterium abscessus by inhibiting enzymes involved in many physiological processes

Project description:Mycobacterium abscessus is nowadays under the spotlight of the scientific community. This pathogenic mycobacteria is indeed responsible for a wide spectrum of infections involving mostly pulmonary infections in patients with cystic fibrosis. M. abscessus is intrinsically resistant to a broad range of antibiotics, including most antitubercular drugs, and is considered the most pathogenic and chemotherapy-resistant rapidly growing mycobacterium. Consequently, with very limited treatment options, the development of new therapeutic approaches to fight this pathogen are urgently needed. 38 new analogs of Cyclipostins & Cyclophostin (CyC), compounds naturally produced by Streptomyces species, have been synthesized. Their antibacterial activities against clinical isolates belonging to the M. chelonae-abscessus clade, as well as Gram-negative and Gram-positive bacteria have been evaluated by the REMA method. The intracellular activities of the CyC against intramacrophagic M. abscessus have also been investigated and compared to those of imipenem. The CyCs displayed very low toxicity towards host cells and their inhibitory activity was exclusively restricted to mycobacteria. The best candidate, CyC17, showed a high selectivity for mycobacteria with MIC values (<2 up to 40 µg/mL) comparable to those of most classical antibiotics used to treat M. abscessus infections. Of importance, several CyCs were active against extracellular M. abscessus growth (i.e., CyC17 / CyC18β / CyC25 / CyC26) or against intracellular mycobacteria inside macrophages (i.e., CyC7α,β / CyC8α,β) with MIC values similar to or better than those of standard antibiotics. Based on these results, we intended to identify the potential target enzymes of CyC17/CyC26 in M. abscessus by activity-based protein profiling (ABPP) approach coupled with mass spectrometry differential analysis.

2019-10-08 | PXD014255 | Pride

Dissecting the antibacterial activity of Oxadiazolone-core derivatives against Mycobacterium abscessus

Project description:Mycobacterium abscessus is nowadays under the spotlight of the scientific community. This pathogenic mycobacteria is indeed responsible for a wide spectrum of infections involving mostly pulmonary infections in patients with cystic fibrosis. M. abscessus is intrinsically resistant to a broad range of antibiotics, including most antitubercular drugs, and is considered the most pathogenic and chemotherapy-resistant rapidly growing mycobacterium. Consequently, with very limited treatment options, the development of new therapeutic approaches to fight this pathogen are urgently needed. In this context, 19 oxadiazolone (OX) derivatives have been investigated for their antibacterial activity against both the rough (R) and smooth (S) variants of M. abscessus. Several OXs were active against extracellular M. abscessus growth with moderated minimal inhibitory concentrations (MIC), or intracellularly by inhibiting M. abscessus growth inside infected macrophages with MIC values similar to those of imipenem. Such promising results prompted us to identify the potential target enzymes of the sole extra and intracellular inhibitor of M. abscessus growth, i.e., iBpPPOX via activity-based protein profiling combined with mass spectrometry. This approach led to the identification of 21 potential protein candidates being mostly involved in M. abscessus lipid metabolism and/or in cell wall biosynthesis.

2020-08-14 | PXD015680 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data