Proteomics

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Vascular rays mediate radial assimilate transport in tuberous roots of Manihot esculenta


ABSTRACT: Cassava (Manihot esculenta) is one of the most important staple food crops worldwide. Its starchy tuberous roots supply over 800 million people with carbohydrates. Yet, surprisingly little is known about the processes involved in filling of those vital storage organs. A better understanding of cassava carbohydrate allocation and starch storage is key to improve storage root yield. In this work, we studied cassava morphology and phloem sap flow from source to sink using transgenic pAtSUC2::GFP plants, the phloem tracers esculin and 5(6)-carboxyfluorescein diacetate (CFDA), as well as several staining techniques. We show that cassava performs apoplasmic phloem loading in source leaves and symplasmic unloading into phloem parenchyma cells of tuberous roots. We demonstrate that vascular rays play an important role in radial transport from the phloem to xylem parenchyma cells in tuberous roots. Furthermore, enzymatic and proteomic measurements of storage root tissues confirmed high abundance and activity of enzymes involved in the sucrose synthase-mediated pathway and indicated that starch is stored most efficiently in the outer xylem layers of tuberous roots. Our findings represent a first basis for biotechnological approaches aimed at improved phloem loading and enhanced carbohydrate allocation and storage in order to increase tuberous root yield of cassava.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Manihot Esculenta (cassava) (jatropha Manihot)

TISSUE(S): Root

SUBMITTER: Christian Lamm  

LAB HEAD: Wolfgang Zierer

PROVIDER: PXD014001 | Pride | 2019-07-08

REPOSITORIES: Pride

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Publications

Symplasmic phloem unloading and radial post-phloem transport via vascular rays in tuberous roots of Manihot esculenta.

Mehdi Rabih R   Lamm Christian E CE   Bodampalli Anjanappa Ravi R   Müdsam Christina C   Saeed Muhammad M   Klima Janine J   Kraner Max E ME   Ludewig Frank F   Knoblauch Michael M   Gruissem Wilhelm W   Sonnewald Uwe U   Zierer Wolfgang W  

Journal of experimental botany 20191001 20


Cassava (Manihot esculenta) is one of the most important staple food crops worldwide. Its starchy tuberous roots supply over 800 million people with carbohydrates. Yet, surprisingly little is known about the processes involved in filling of those vital storage organs. A better understanding of cassava carbohydrate allocation and starch storage is key to improving storage root yield. Here, we studied cassava morphology and phloem sap flow from source to sink using transgenic pAtSUC2::GFP plants,  ...[more]

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