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Proteomics profiling of Zika- or HIV-infected moncoytes

ABSTRACT: human primary monocytes purified from 2 healthy blood donors were infected in vitro with Zika virus or HIV for 48 hours. Proteomics profiling of infected versus non infected cells was performed to identify up/down-regulated proteins associated to the infection

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Monocyte

DISEASE(S): Zika Virus Congenital Syndrome,Human Immunodeficiency Virus Infectious Disease

SUBMITTER: Francois Delalande

LAB HEAD: Sarah CIANFERANI

PROVIDER: PXD014002 | Pride | 2019-08-22

REPOSITORIES: Pride

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Zika virus enhances monocyte adhesion and transmigration favoring viral dissemination to neural cells.

Ayala-Nunez Nilda Vanesa NV Follain Gautier G Delalande François F Hirschler Aurélie A Partiot Emma E Hale Gillian L GL Bollweg Brigid C BC Roels Judith J Chazal Maxime M Bakoa Florian F Carocci Margot M Bourdoulous Sandrine S Faklaris Orestis O Zaki Sherif R SR Eckly Anita A Uring-Lambert Béatrice B Doussau Frédéric F Cianferani Sarah S Carapito Christine C Jacobs Frank M J FMJ Jouvenet Nolwenn N Goetz Jacky G JG Gaudin Raphael R

Nature communications 20190927 1

Zika virus (ZIKV) invades and persists in the central nervous system (CNS), causing severe neurological diseases. However the virus journey, from the bloodstream to tissues through a mature endothelium, remains unclear. Here, we show that ZIKV-infected monocytes represent suitable carriers for viral dissemination to the CNS using human primary monocytes, cerebral organoids derived from embryonic stem cells, organotypic mouse cerebellar slices, a xenotypic human-zebrafish model, and human fetus b ...[more]

PMID: 31562326

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