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MTOR-regulated phosphorylation of electrogenic sodium bicarbonate cotransporter 1, NBCe1, at S255-257 regulates NBCe1 functional expression in mouse cortical astrocyte

ABSTRACT: The electrogenic sodium bicarbonate cotransporter 1, NBCe1 (SLC4A4), is the major bicarbonate transporter expressed in astrocytes. It is highly sensitive for bicarbonate, and the main regulator of intracellular-, extracellular-, and synaptic pH, thereby modulating neuronal excitability. However, despite these essential functions, the molecular mechanisms underlying NBCe1-mediated astrocytic response to extracellular pH changes are mostly unknown. Using primary cortical astrocyte cultures we have investigated the effect of long-term extracellular metabolic alkalosis on regulation of NBCe1 using functional assays and phosphoproteomic analysis.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Astrocyte Of The Cerebral Cortex

SUBMITTER: Jana Zecha

LAB HEAD: Bernhard Kuster

PROVIDER: PXD014034 | Pride | 2019-07-15

REPOSITORIES: Pride

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			Action	DRS
	02941_BE3_P033643_B00_A00_R1.raw	Raw
	02941_BF3_P033644_B00_A00_R1.raw	Raw
	02941_BG3_P033645_B00_A00_R1.raw	Raw
	02941_BH3_P033646_B00_A00_R1.raw	Raw
	02982_GA4_P034211_B00_A00_R1.raw	Raw

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