Proteomics

Dataset Information

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The exploration of Missing Proteins by a combination approach to enrich the low abundance membrane proteins from several cancer cell lines


ABSTRACT: Our goal is the identification of more and more the missing proteins from cancer cell lines. This project focuses on the membrance proteins separated by the ultracentrifuge and the low abundant peptides enriched by the Proteominer Kit and the hydrophobic peptides extracted by the organic solvent Acetonitrile.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Colon Cancer,Cervix Carcinoma

SUBMITTER: yuanliang zhang  

LAB HEAD: Siqi Liu

PROVIDER: PXD014058 | Pride | 2019-12-06

REPOSITORIES: Pride

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Publications

Exploration of Missing Proteins by a Combination Approach to Enrich the Low-Abundance Hydrophobic Proteins from Four Cancer Cell Lines.

Zhang Yuanliang Y   Lin Zhilong Z   Tan Yifan Y   Bu Fanyu F   Hao Piliang P   Zhang Keren K   Yang Huanming H   Liu Siqi S   Ren Yan Y  

Journal of proteome research 20191210 1


The mission of the Chromosome-Centric Human Proteome Project (C-HPP) to discover missing proteins (MPs) has become increasingly difficult due to the remaining low-abundance, high-hydrophobicity, or low-molecular-weight MPs. We have reported two approaches to resolve these identification problems for the low-abundance and high-hydrophobicity MPs, respectively. In this study, to improve the identification of low-abundance MPs with high hydrophobicity, we combined two approaches and obtained MPs fr  ...[more]

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