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Proteomics analysis of pancreatic cancer cell line (MiaPaCa-2)

ABSTRACT: Project describes the proteomics analysis using pSILAC approach to identify and quantify the hypoxia induced protein under serum and serum free conditions in pancreatic cancer cell line (MiaPaCa-2)

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Pancreatic Cancer

SUBMITTER: Nikhil Gupta

LAB HEAD: Newman Sze Siu Kwan

PROVIDER: PXD014087 | Pride | 2019-11-13

REPOSITORIES: Pride

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ERO1α promotes hypoxic tumor progression and is associated with poor prognosis in pancreatic cancer.

Gupta Nikhil N Park Jung Eun JE Tse Wilford W Low Jee Keem JK Kon Oi Lian OL McCarthy Neil N Sze Siu Kwan SK

Oncotarget 20191015 57

Pancreatic cancer is a leading cause of mortality worldwide due to the difficulty of detecting early-stage disease and our poor understanding of the mediators that drive progression of hypoxic solid tumors. We therefore used a heavy isotope 'pulse/trace' proteomic approach to determine how hypoxia (Hx) alters pancreatic tumor expression of proteins that confer treatment resistance, promote metastasis, and suppress host immunity. Using this method, we identified that hypoxia stress stimulates pan ...[more]

PMID: 31666928

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