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The RNA fold interactome of evolutionary conserved yeast RNA structures in S. cerevisiae

ABSTRACT: Based on previous DMS-Seq data that provides in vivo structural conformation of RNA, we here applied a streamlined RNA pull-down approach to study 186 evolutionary conserved RNA folds in S. cerevisiae. These folds bound 162 different proteins, with the majority known or inferred to be RNA-binding proteins. In contrast to global RNA interactome capture methods; we were able to associate individual messenger RNAs harboring conserved RNA folds with their set of bound regulating proteins. Our data suggests a more widespread function of tRNA synthetases as mRNA regulators. Using an in vivo RNA reporter screen, we show that specific mRNA-RBP pairs regulate protein expression, which connects structural RNA features recognized by the RNA-binding protein to functionality.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: F Butter

LAB HEAD: Falk Butter

PROVIDER: PXD014092 | Pride | 2020-07-02

REPOSITORIES: Pride

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The RNA fold interactome of evolutionary conserved RNA structures in S. cerevisiae.

Casas-Vila Nuria N Sayols Sergi S Pérez-Martínez Lara L Scheibe Marion M Butter Falk F

Nature communications 20200603 1

RNA-binding proteins play key roles in regulation of gene expression via recognition of structural features in RNA molecules. Here we apply a quantitative RNA pull-down approach to 186 evolutionary conserved RNA structures and report 162 interacting proteins. Unlike global RNA interactome capture, we associate individual RNA structures within messenger RNA with their interacting proteins. Of our binders 69% are known RNA-binding proteins, whereas some are previously unrelated to RNA binding and ...[more]

PMID: 32493961

Publication: 1/2

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