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Age-dependent dynamics of ribosomal proteins in mouse brain

ABSTRACT: To determine the dynamics of ribosomal proteins in brain tissues, we isolated monoribosomes and ribosomes engaged in polysomal fractions from three different brain tissues, cerebellum, hippocampus and cortex, and compared them to the liver of CD-1 IGS mouse. We provide a comprehensive unique study of the ribosomal composition among these different tissues along different life stages, from juvenile (three-weeks), young adult (four-months) to mature adults (seven- and twelve-months) of age. The protein composition of the ribosomes was quantified using LC-MS/MS approach.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Christoph Krisp

LAB HEAD: Zoya Ignatova

PROVIDER: PXD014138 | Pride | 2019-10-10

REPOSITORIES: Pride

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Invariable stoichiometry of ribosomal proteins in mouse brain tissues with aging.

Amirbeigiarab Susan S Kiani Parnian P Velazquez Sanchez Ana A Krisp Christoph C Kazantsev Andriy A Fester Lars L Schlüter Hartmut H Ignatova Zoya Z

Proceedings of the National Academy of Sciences of the United States of America 20191021 45

Across phyla, the ribosomes-the central molecular machines for translation of genetic information-exhibit an overall preserved architecture and a conserved functional core. The natural heterogeneity of the ribosome periodically phases a debate on their functional specialization and the tissue-specific variations of the ribosomal protein (RP) pool. Using sensitive differential proteomics, we performed a thorough quantitative inventory of the protein composition of ribosomes from 3 different mouse ...[more]

PMID: 31636180

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