Dataset Information

The mechanism of infochemicals induced colony formation and flocculation in the microalga Scenedesmus subspicatus

ABSTRACT: Daphnia infochemicals can induce defensive responses in microalgae Scenedesmus spp., including colony formation and flocculation. We investigated the phenomenon using a combination of microalgal biology, physiological ecology, and quantitative isobaric tagging (iTRAQ) proteomics. We have discovered that flocculation as a defense response occurs at the early ‘alarm’ phase and requires increased energy resources, with an important role proposed in cysteine synthesis. It appears to be initially stimulated by the production of an extracellular matrix (mainly composed ofby polysaccharides and fatty acids), and later sustained at the ‘acclimation’ stage through Mitogen-activated protein kinase (MAPK) signalling cascades. Defensive strategies leading to colony formation required investment into fatty acids metabolism, to ensure the accurate separation of membranes during cell division. Furthermore, higher energy demands were required at the alarm phase which subsequently decreased at the acclimation stage, thus suggesting a trade-off between colony formation and support of floc form.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Desmodesmus Subspicatus

TISSUE(S): Cell Suspension Culture

SUBMITTER: Sebastiana Roccuzzo

LAB HEAD: Jagroop Pandhal

PROVIDER: PXD014153 | Pride | 2020-05-06

REPOSITORIES: Pride

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Publications

Metabolic Insights Into Infochemicals Induced Colony Formation and Flocculation in Scenedesmus subspicatus Unraveled by Quantitative Proteomics.

Roccuzzo Sebastiana S Couto Narciso N Karunakaran Esther E Kapoore Rahul Vijay RV Butler Thomas O TO Mukherjee Joy J Hansson Erika M EM Beckerman Andrew P AP Pandhal Jagroop J

Frontiers in microbiology 20200507

Microalgae can respond to natural cues from crustacean grazers, such as Daphnia, by forming colonies and aggregations called flocs. Combining microalgal biology, physiological ecology, and quantitative proteomics, we identified how infochemicals from Daphnia trigger physiological and cellular level changes in the microalga Scenedesmus subspicatus, underpinning colony formation and flocculation. We discovered that flocculation occurs at an energy-demanding 'alarm' phase, with ...[more]

PMID: 32457714

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Dataset Information

The mechanism of infochemicals induced colony formation and flocculation in the microalga Scenedesmus subspicatus

Publications

Metabolic Insights Into Infochemicals Induced Colony Formation and Flocculation in <i>Scenedesmus subspicatus</i> Unraveled by Quantitative Proteomics.

Similar Datasets

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