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Determination of GPI structure of human CD59 in HEK293 cells

ABSTRACT: To determine GPI structure of human CD59 in wild-type,B3GALT4-KO, PIGZ-B3GALT4-DKO HEK293 cells.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Epithelial Cell

SUBMITTER: Yicheng Wang

LAB HEAD: Taroh Kinoshita

PROVIDER: PXD014226 | Pride | 2020-02-17

REPOSITORIES: Pride

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Cross-talks of glycosylphosphatidylinositol biosynthesis with glycosphingolipid biosynthesis and ER-associated degradation.

Wang Yicheng Y Maeda Yusuke Y Liu Yi-Shi YS Takada Yoko Y Ninomiya Akinori A Hirata Tetsuya T Fujita Morihisa M Murakami Yoshiko Y Kinoshita Taroh T

Nature communications 20200213 1

Glycosylphosphatidylinositol (GPI)-anchored proteins and glycosphingolipids interact with each other in the mammalian plasma membranes, forming dynamic microdomains. How their interaction starts in the cells has been unclear. Here, based on a genome-wide CRISPR-Cas9 genetic screen for genes required for GPI side-chain modification by galactose in the Golgi apparatus, we report that β1,3-galactosyltransferase 4 (B3GALT4), the previously characterized GM1 ganglioside synthase, additionally functio ...[more]

PMID: 32054864

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