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Signature-Ion Triggered Mass Spectrometry Approach Enabled Discovery of N- and O-linked Glycosylated Neuropeptides in the Crustacean Nervous System

ABSTRACT: Discovery of N-linked and O-linked glycosylation on crustacean neuropeptides.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Callinectes Sapidus Cancer Irroratus

TISSUE(S): Brain, Thoracic Ganglion

SUBMITTER: Qinjingwen Cao

LAB HEAD: Qinjingwen Cao

PROVIDER: PXD014269 | Pride | 2021-09-08

REPOSITORIES: Pride

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Signature-Ion-Triggered Mass Spectrometry Approach Enabled Discovery of N- and O-Linked Glycosylated Neuropeptides in the Crustacean Nervous System.

Cao Qinjingwen Q Yu Qing Q Liu Yang Y Chen Zhengwei Z Li Lingjun L

Journal of proteome research 20200113 2

Crustaceans are commonly used model organisms to study neuromodulation. Despite numerous reported crustacean neuropeptide families and their functions, there has been no report on neuropeptide glycosylation. This is in part due to a lack of sensitive methods that enable deciphering this intricate low-abundance post-translational modification, even though glycosylation has been shown to play an important role in neuromodulation. Here, we describe the discovery of glycosylated neuropeptides with a ...[more]

PMID: 31875397

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