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QUANTITATIVE SUBCELLULAR PROTEOMICS OF THE ORBITOFRONTAL CORTEX OF SCHIZOPHRENIA PATIENTS

ABSTRACT: We analyzed orbitofrontal cortex post-mortem brain tissue from patients. We generated an organellar proteome map from different subcellular fractions such as mitochondria (MIT), crude nuclear fraction (NUC) and cytoplasm (CYT) with the aim to monitor the subproteome changes using iTRAQ labelling for relative quantification and Selected Reaction Monitoring (SRM) for targeted quantification.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain

DISEASE(S): Schizophrenia

SUBMITTER: Proteomics Unit

LAB HEAD: Fabio Nogueira

PROVIDER: PXD014284 | Pride | 2019-10-10

REPOSITORIES: Pride

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Quantitative Subcellular Proteomics of the Orbitofrontal Cortex of Schizophrenia Patients.

Velásquez Erika E Martins-de-Souza Daniel D Velásquez Ingrid I Carneiro Gabriel Reis Alves GRA Schmitt Andrea A Falkai Peter P Domont Gilberto B GB Nogueira Fabio C S FCS

Journal of proteome research 20191028 12

Schizophrenia is a chronic disease characterized by the impairment of mental functions with a marked social dysfunction. A quantitative proteomic approach using iTRAQ labeling and SRM, applied to the characterization of mitochondria (MIT), crude nuclear fraction (NUC), and cytoplasm (CYT), can allow the observation of dynamic changes in cell compartments providing valuable insights concerning schizophrenia physiopathology. Mass spectrometry analyses of the orbitofrontal cortex from 12 schizophre ...[more]

PMID: 31581776

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