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One-pot analysis on cyclosporine A binding

ABSTRACT: Investigate if one-pot analysis works on several energetic-based proteomics method on cyclosporine A.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Baiyi Quan

LAB HEAD: Michael C. Fitzgerald

PROVIDER: PXD014309 | Pride | 2021-09-08

REPOSITORIES: Pride

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Comparative Analysis of Mass-Spectrometry-Based Proteomic Methods for Protein Target Discovery Using a One-Pot Approach.

Cabrera Aurora A Wiebelhaus Nancy N Quan Baiyi B Ma Renze R Meng He H Fitzgerald Michael C MC

Journal of the American Society for Mass Spectrometry 20191122 2

Recently, several mass-spectrometry- and protein-denaturation-based proteomic methods have been developed to facilitate protein target discovery efforts in drug mode-of-action studies. These methods, which include the stability of proteins from rates of oxidation (SPROX), pulse proteolysis (PP), chemical denaturation and protein precipitation (CPP), and thermal proteome profiling (TPP) techniques, have been used in an increasing number of applications in recent years. However, while the advantag ...[more]

PMID: 32031398

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