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Proteomic-based identification of oocyte maturation-related proteins in mouse GV oocytes

ABSTRACT: In this study, we applied 1D SDS-PAGE and RP-LC-MS/MS to investigate the proteins stored in GV mouse oocytes. This high-performance strategy allowed us to define a set of 1405 different mouse GV oocyte proteins. It is confirmed that this study will help us to understand the diverse biological processes occurring in mouse oocytes and during early embryo development. However, compared with proteomic analysis of other cells and tissues, such as embryonic stems from cells and liver, the proteins identified in mature mouse oocytes were limited. This was mainly due to the fact that oocytes obtained from each mouse were very limited. We believe that the catalog of maternal proteins presented in this article is a starting point and we anticipate that more researches on the oocyte proteome will deduce most of the maternal proteins.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Oocyte

SUBMITTER: Senyang Cao

LAB HEAD: Senyang Cao

PROVIDER: PXD014314 | Pride | 2020-09-17

REPOSITORIES: Pride

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Proteomic-based identification of oocyte maturation-related proteins in mouse germinal vesicle oocytes.

Cao Senyang S Huang Shaoping S Guo Ying Y Zhou Lin L Lu Ying Y Lai Shanshan S

Reproduction in domestic animals = Zuchthygiene 20201014 11

Oocyte proteins play an important role in oocyte maturation, fertilization and embryonic development. However, the protein composition of mouse germinal vesicle (GV) oocytes is still unclear. Using one-dimensional Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (1D SDS-PAGE) and Reverse-phase liquid chromatography tandem mass spectrometry (RP-LC-MS/MS), we constructed a protein profile of mouse GV oocytes. First, our proteomics profile identified 1,405 different proteins from 11,000 m ...[more]

PMID: 32920902

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