Dataset Information

Qualitative and label-free quantitative analysis of protein changes in the lymphatic muscle cells isolated in a hind-limb lymphatic injury model in the sheep

ABSTRACT: We developed a large animal model, with clinically relevant gravitational loading on the lymphatic vasculature, that enables longitudinal tracking of lymphatic function in vivo as well as end-point analysis of vessel function and mechanics. One of two parallel lymphatic vessels in the sheep hind limb was ligated with the contralateral limb acting as an internal control. To determine the changes in the protein expression profiles in the lymphatic muscle cells from the remodeled vessel compared to the control, we expanded isolated LMC in vitro and performed bottom-up proteomic analysis using a Q Exactive Plus mass spectrometer. Label-free quantitative (LFQ) methods directly used the raw spectral data from parallel MS runs to determine relative protein abundances. We used both “MS/MS (MS2) spectral counting” and “precursor MS1 area” methods for label free quantitation and further contrasting the differentially expressed proteomic profiles across the control and remodeled lymphatic muscle samples to determine the alterations in mitochondrial dysfunction and elevated oxidative stress within the lymphatic muscle.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Ovis Aries

TISSUE(S): Lymphatic Endothelium, Smooth Muscle Tissue

DISEASE(S): Disease Free

SUBMITTER: Cristina Clement

LAB HEAD: Brandon Dixon PhD

PROVIDER: PXD014343 | Pride | 2021-01-12

REPOSITORIES: Pride

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Publications

Lymphatic remodelling in response to lymphatic injury in the hind limbs of sheep.

Nelson Tyler S TS Nepiyushchikh Zhanna Z Hooks Joshua S T JST Razavi Mohammad S MS Lewis Tristan T Clement Cristina C CC Thoresen Merrilee M Cribb Matthew T MT Ross Mindy K MK Gleason Rudolph L RL Santambrogio Laura L Peroni John F JF Dixon J Brandon JB

Nature biomedical engineering 20191223 6

Contractile activity in the lymphatic vasculature is essential for maintaining fluid balance within organs and tissues. However, the mechanisms by which collecting lymphatics adapt to changes in fluid load and how these adaptations influence lymphatic contractile activity are unknown. Here we report a model of lymphatic injury based on the ligation of one of two parallel lymphatic vessels in the hind limb of sheep and the evaluation of structural and functional changes in the intact, remodelling ...[more]

PMID: 31873209

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Dataset Information

Qualitative and label-free quantitative analysis of protein changes in the lymphatic muscle cells isolated in a hind-limb lymphatic injury model in the sheep

Publications

Lymphatic remodelling in response to lymphatic injury in the hind limbs of sheep.

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