Proteomics

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Biogenesis and DNA cargo of Dinoroseobacter shibae outer membrane vesicles suggests vesicle formation is coupled to replication


ABSTRACT: The Gram-negative photoheterotrophic bacterium Dinoroseobacter shibae is a member of the high abundant marine Roseobacter group. In the ocean, OMVs have about the same abundance as bacteria, a distinct depth distribution, and contain DNA from a variety of marine bacterial taxa. In the present study, we determined the abundance, size, and ultrastructure of membrane vesicles of D. shibae and analysed the protein inventory of the soluble and membrane fractions of cells and vesicles in order to study the origin of OMV membranes and content. The proteomic analyses were complemented by fatty acid analyses.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Dinoroseobacter Shibae (strain Dfl 12)

SUBMITTER: Martin Kucklick  

LAB HEAD: Susanne Engelmann

PROVIDER: PXD014351 | Pride | 2020-12-16

REPOSITORIES: Pride

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