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Candida albicans (ATCC90028 and ATCC10231 yeast) extracellular vesicle and whole cell lysate proteomes

ABSTRACT: We performed proteomic analysis of extracellular vesicles (EVs) and whole cell lysates (WCL) isolated from the same liquid culture. This was implemented for two different C. albicans strains, ATCC90028 (n = 3) and ATCC10231 (n = 3). This data set is the first label-free quantitative comparative study of the proteins in C. albicans EVs and their parent planktonic (yeast-form) cells. We identified proteins enriched in EVs versus WCL by comparing their LFQ intensities across all samples. After linking these results with EV enrichment data from other strains, we proposed a suite of putative EV marker proteins which were useful for multiple C. albicans strains and morphologies.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Candida Albicans (yeast)

SUBMITTER: Charlotte Dawson

LAB HEAD: Mark Bleackley

PROVIDER: PXD014388 | Pride | 2020-04-17

REPOSITORIES: Pride

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Protein markers for <i>Candida albicans</i> EVs include claudin-like Sur7 family proteins.

Dawson Charlotte S CS Garcia-Ceron Donovan D Rajapaksha Harinda H Faou Pierre P Bleackley Mark R MR Anderson Marilyn A MA

Journal of extracellular vesicles 20200416 1

<b>Background:</b> Fungal extracellular vesicles (EVs) have been implicated in host-pathogen and pathogen-pathogen communication in some fungal diseases. In depth research into fungal EVs has been hindered by the lack of specific protein markers such as those found in mammalian EVs that have enabled sophisticated isolation and analysis techniques. Despite their role in fungal EV biogenesis, ESCRT proteins such as Vps23 (Tsg101) and Bro1 (ALIX) are not present as fungal EV cargo. Furthermore, tet ...[more]

PMID: 32363014

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