Dataset Information

High-pH Reversed-Phase Fractionated Neural Retina Proteome of Normal Growing C57BL/6 Mouse

ABSTRACT: Mouse was sacrificed at postnatal day 46 by cervical dislocation. Retina were prepared seamlessly with suspension trap (S-TRAP) followed by high-pH reversed-phase peptide fractionation. Peptides fractions were identified with data-dependent acquisition by quadrupole time-of-flight mass spectrometry.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Retina

SUBMITTER: Chuen Lam

LAB HEAD: Chuen Lam

PROVIDER: PXD014459 | Pride | 2019-09-11

REPOSITORIES: Pride

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High-pH reversed-phase fractionated neural retina proteome of normal growing C57BL/6 mouse.

Sze Ying Hon YH Zhao Qian Q Cheung Jimmy Ka Wai JKW Li King Kit KK Tse Dennis Yan Yin DYY To Chi Ho CH Lam Thomas Chuen TC

Scientific data 20210126 1

The retina is a key sensory tissue composed of multiple layers of cell populations that work coherently to process and decode visual information. Mass spectrometry-based proteomics approach has allowed high-throughput, untargeted protein identification, demonstrating the presence of these proteins in the retina and their involvement in biological signalling cascades. The comprehensive wild-type mouse retina proteome was prepared using a novel sample preparation approach, the suspension trapping ...[more]

PMID: 33500412

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