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C. elegans wild type compared to vhl-1(ok161) RBPome and Proteome

ABSTRACT: We performed RNA interactome capture in wild type compared to vhl-1(ok161) loss-of-function worms. These data are combined with the proteome to distinguish changes in RBP abundance from differential binding events.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Caenorhabditis Elegans

SUBMITTER: Francesca Fabretti

LAB HEAD: Bernhard Schermer

PROVIDER: PXD014469 | Pride | 2020-01-08

REPOSITORIES: Pride

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Activation of Hypoxia-Inducible Factor Signaling Modulates the RNA Protein Interactome in Caenorhabditis elegans.

Esmaillie Reza R Ignarski Michael M Bohl Katrin K Krüger Tim T Ahmad Daniyal D Seufert Lisa L Schermer Bernhard B Benzing Thomas T Müller Roman-Ulrich RU Fabretti Francesca F

iScience 20191127

The cellular response to hypoxia is crucial to organismal survival, and hypoxia-inducible factors (HIF) are the key mediators of this response. HIF-signaling is central to many human diseases and mediates longevity in the nematode. Despite the rapidly increasing knowledge on RNA-binding proteins (RBPs), little is known about their contribution to hypoxia-induced cellular adaptation. We used RNA interactome capture (RIC) in wild-type Caenorhabditis elegans and vhl-1 loss-of-function mutants to fi ...[more]

PMID: 31835171

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IP of 5-hydroxymethylcytosine (5-hmC) enriched DNA fragments from control ,PB treated mouse livers , resulting Ctnnb1 mutated PB liver tumours and PB minus Ha-Ras mutate liver tumour

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