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Interactome of overexpressed BAG3 in HEK293T cells

ABSTRACT: HEK293T cells stably expressing N-terminally HA-tagged BAG3 were employed to screen for novel BAG3 interacting proteins.

INSTRUMENT(S): LTQ

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hepatocyte, Epithelial Cell, Kidney

SUBMITTER: Christian Behrends

LAB HEAD: Christian Behrends

PROVIDER: PXD014473 | Pride | 2019-10-10

REPOSITORIES: Pride

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The Hippo network kinase STK38 contributes to protein homeostasis by inhibiting BAG3-mediated autophagy.

Klimek Christina C Jahnke Ricarda R Wördehoff Judith J Kathage Barbara B Stadel Daniela D Behrends Christian C Hergovich Alexander A Höhfeld Jörg J

Biochimica et biophysica acta. Molecular cell research 20190719 10

Chaperone-assisted selective autophagy (CASA) initiated by the cochaperone Bcl2-associated athanogene 3 (BAG3) represents an important mechanism for the disposal of misfolded and damaged proteins in mammalian cells. Under mechanical stress, the cochaperone cooperates with the small heat shock protein HSPB8 and the cytoskeleton-associated protein SYNPO2 to degrade force-unfolded forms of the actin-crosslinking protein filamin. This is essential for muscle maintenance in flies, fish, mice and men. ...[more]

PMID: 31326538

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