Proteomics

Dataset Information

0

Proteomics of an artificial mixture of three microorganisms to evaluate sample preparation methods for tandem mass spectrometry proteotyping

ABSTRACT: This project provides tandem mass spectrometry datasets of an artificial mixture used to optimize the lysis and evaluate digestion protocols for a fast preparation of peptides for tandem mass spectrometry proteotyping. The microorganism mixture is composed of Bacillus subtilis subsp. spizizenii ATCC 6633, Escherichia coli BL21-Gold(DE3)pLysS AG and Saccharomyces cerevisiae S288C.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Escherichia Coli Bacillus Subtilis Subsp. Spizizenii Atcc 6633 Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Jean ARMENGAUD  

LAB HEAD: Jean ARMENGAUD

PROVIDER: PXD014505 | Pride | 2019-09-30

REPOSITORIES: Pride

Json Xml
altmetric image

Publications

Evaluation of Sample Preparation Methods for Fast Proteotyping of Microorganisms by Tandem Mass Spectrometry.

Hayoun Karim K   Gouveia Duarte D   Grenga Lucia L   Pible Olivier O   Armengaud Jean J   Alpha-Bazin Béatrice B  

Frontiers in microbiology 20190906

Tandem mass spectrometry-based proteotyping allows characterizing microorganisms in terms of taxonomy and is becoming an important tool for investigating microbial diversity from several ecosystems. Fast and automatable sample preparation for obtaining peptide pools amenable to tandem mass spectrometry is necessary for enabling proteotyping as a high-throughput method. First, the protocol to increase the yield of lysis of several representative bacterial and eukaryotic microorganisms was optimiz  ...[more]

Similar Datasets

Unknown Time series of Escherichia coli BL21(DE3) after temperature upshift from 37°C to 42°C, tiling arrays
2020-04-12 | GSE148034 | GEO
Unknown Deciphering the transcriptional changes in Escherichia coli strains C41(DE3) and C43(DE3) that makes them a superior choice for membrane protein production.
2022-06-23 | GSE153028 | GEO
Proteomics High-throughput proteotyping based on microplate paramagnetic beads and phylopeptidomics
2022-04-23 | PXD017720 | Pride
Proteomics ASTRAL deep-proteotyping of a human fecal standard to evaluate metaproteomics strategies.
2024-03-07 | PXD045838 | Pride
Unknown Time series of Escherichia coli BL21(DE3) growth curve, tiling arrays
2017-01-13 | GSE93565 | GEO
Genomics
| PRJNA741995 | ENA
Unknown Microaerobic fermentation alters lactose metabolism in Escherichia coli
2020-05-18 | GSE135698 | GEO
Genomics Strain-dependent Genetic Heterogeneity Shapes Long-term Stability of Bioproduction in E. coli
2020-11-17 | E-MTAB-9800 | biostudies-arrayexpress
Genomics Escherichia coli strain:BL21 (DE3)
| PRJNA520351 | ENA
Genomics DNA-seq of E. coli BL21 (DE3) Evolved in High pH (pH = 9) Terrific Broth Media
2023-10-31 | E-MTAB-12334 | biostudies-arrayexpress