Proteomics

Dataset Information

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The secretome of the rumen fluke, Calicophoron daubneyi

ABSTRACT: Helminth parasites secrete molecules in soluble form (i.e the secretome) or packaged into extracellular vesicles (EVs) as a means of exporting effector molecules into the host microenvironment. Once released, the parasite-derived molecules can trigger a range of biological effects including modulation of host immunity. While the secretome and molecular cargo of EVs have been characterised in many parasites, little is known about the molecules released by the rumen fluke, Calicophoron daubneyi – an emerging infection of livestock in Western Europe. Here we used a mass spectrometry-based proteomics approach to characterise the secretome of the infective newly-excysted juvenile (NEJ) stage and adult flukes. We also profile the proteome of two sub-populations of EVs (termed 15K and 120K EVs) released by the adult flukes.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Platyhelminthes Calicophoron Daubneyi

TISSUE(S): Multicellular Organism, Secretion

SUBMITTER: Mark Robinson  

LAB HEAD: Mark W. Robinson

PROVIDER: PXD014550 | Pride | 2021-02-16

REPOSITORIES: Pride

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Publications

Transcriptome and Secretome Analysis of Intra-Mammalian Life-Stages of Calicophoron daubneyi Reveals Adaptation to a Unique Host Environment.

Huson Kathryn M KM   Atcheson Erwan E   Oliver Nicola A M NAM   Best Philip P   Barley Jason P JP   Hanna Robert E B REB   McNeilly Tom N TN   Fang Yongxiang Y   Haldenby Sam S   Paterson Steve S   Robinson Mark W MW  

Molecular & cellular proteomics : MCP 20210211

Paramphistomosis, caused by the rumen fluke, Calicophoron daubneyi, is a parasitic infection of ruminant livestock, which has seen a rapid rise in prevalence throughout Western Europe in recent years. After ingestion of metacercariae (parasite cysts) by the mammalian host, newly excysted juveniles (NEJs) emerge and invade the duodenal submucosa, which causes significant pathology in heavy infections. The immature flukes then migrate upward, along the gastrointestinal tract, and enter the rumen w  ...[more]

PMID: 33581320

Publication: 1/2

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