Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Palaeoproteomic analysis of ancient eggshell proteins from Çatalhöyük archaeological site

ABSTRACT: Palaeoproteomics analysis of archaeological bird eggshells from archaeological Çatalhöyük site. This project aimed to taxonomically identify fragments of eggshells from the site, using a combination of morphological observations (by light microscopy) and protein analyses (MALDI-MS and LC-MS/MS).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Aves

TISSUE(S): Eggshell

SUBMITTER: Beatrice Demarchi

LAB HEAD: Beatrice Demarchi

PROVIDER: PXD014558 | Pride | 2020-06-10

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Similar Datasets

Ancient proteins resolve controversy over the identity of Genyornis eggshell

Project description:Here we show the potential of proteins preserved in Pleistocene eggshell for addressing a longstanding controversy in human and evolution: the identity of the extinct bird that laid the eggs which were exploited by Australia’s first inhabitants. The eggs had been originally attributed to the iconic extinct flightless Genyornis newtoni, and subsequently dated to before 50 ±5 ka by Miller et al. (2016). This was taken to represent the extinction date for this endemic megafaunal species and thus implied a role of humans in its demise. A contrasting hypothesis, according to which the eggshell was laid by a large megapode (mound-builder), would therefore acquit humans of their responsibility in the extinction of Genyornis. Ancient protein sequences were reconstructed and used to assess the evolutionary proximity of the undetermined eggshell to extant birds, rejecting the megapode hypothesis. Ancient DNA could not be retrieved from these highly degraded samples, but morphometric data supported the attribution of the eggshell to Genyornis. When used in triangulation to address well-defined hypotheses, palaeoproteomics is a precious tool for reconstructing the evolutionary history of extinct and extant species. Here we show that the identification of Genyornis eggshell implies a more nuanced understanding of the modes of interactions between humans and their environment.

2022-07-19 | PXD027713 | Pride

Characterisation of eggshell proteomes from the extinct Madagascar elephant bird

Project description:Characterisation of eggshell proteomes from the extinct Madagascar elephant bird

2023-01-20 | PXD035725 | Pride

Mineral-bound ostrich eggshell peptides survive for up to 3.8 Ma in equatorial Africa

Project description:Claims for exceptional preservation of biomolecules in the fossil record are contested. Here we demonstrate the role of surface stabilisation in significantly prolonging protein sequence survival to ~3.8 million years. The intracrystalline environment of calcite ostrich (Struthionidae) eggshell encapsulates uterine proteins and molecular dynamics simulations of struthiocalcin-1 & -2, the dominant proteins within the eggshell, reveal that they bind to the mineral surface in distinct domains. By ~3.8 million years the struthiocalcin-1 domain with the lowest calculated binding energy is selectively preserved in eggshell samples from equatorial Africa. Sequence survival is explained by entropy loss of the peptide and water, lowering the effective temperature of the local environment at the peptide mineral interface.

2018-07-16 | PXD003786 | Pride

Birds Of Prey And Humans In Prehistoric Europe: A View From El Mirón Cave, Cantabria (Spain).

Project description:Paleoproteomics analysis of avian eggshell from the prehistoric cave site of El Mirón.

2019-01-28 | PXD010439 | Pride

Ostrich eggshell peptides survival into the Miocene (6-9 Ma)

Project description:The oldest authenticated peptide sequences to date were reported in 2016 from 3.8 Ma old ostrich eggshell (OES) from the site of Laetoli, Tanzania (Demarchi et al., 2016). Here we demonstrate survival of the same sequences in 6.5-9Ma OES recovered from a palaeosteppe setting in northwestern China. The eggshell is thicker than those observed in extant species and consistent with the Liushu Struthio sp. ootaxon. These findings push the preservation of ancient proteins back to the Miocene and highlight their potential for paleontology, paleoecology and evolutionary biology.

2022-12-29 | PXD035872 | Pride

Aedes aegypti mosquito eggshell proteins in response to RNAi-EOF1

Project description:Aedes aegypti mosquito ovarian follicles develop synchronously after taking a blood meal. A single layer of follicular epithelial cells surrounding the oocyte is responsible for secreting a majority of eggshell structural components between 18 and 54 hours post blood meal. We previously identified a protein called eggshell organizing factor 1 (EOF1). When we knocked down EOF1 with RNA interference in female adult mosquitoes, they laid eggs that were not properly melanized, and fragile eggs did not contain viable embryos. Here, we aimed to determine putative downstream eggshell proteins in RNAi-EOF1 female mosquitoes. Our eggshell proteomics identified 220 proteins, suggesting that mosquito extracellular eggshells are composed of a complex mixture of proteins. These proteins are involved in the formation of an intact eggshell that protects the embryonic development and larva from the environment for long periods of time.

2023-09-29 | PXD045606 | Pride

Eggshell-matrix protein proteome for squamates

Project description:We compared the eggshell proteome of the crocodile lizard (Shinisaurus crocodilurus) to the oviparous Mongolia racerunner (Eremias argus).

2022-08-17 | PXD029080 | Pride

Peptides from archeological chicken eggshell

Project description:MS/MS spectra of archaeological eggshell identified as Gallus gallus

2022-02-07 | MSV000088794 | MassIVE

Characterization and quantification of chicken eggshell proteins during pivotal events of shell calcification

Project description:Gallus gallus avian eggshell is composed of 95% calcium carbonate on calcitic form and of 3.5% extracellular organic matrix (proteins, polysaccharides and proteoglycans). This highly ordered structure with a polycrystalline organization result of the control of mineral deposition by the organic matrix components in the lumen of the uterus. This interaction leads to the eggshell ultrastructure and consequently contributes to its resulting mechanical properties. This study used GeLC MS/MS analyses combined to label free quantitative analysis to identify and quantify matrix proteins at the pivotal step of the calcification process (amorphous calcium carbonate deposit, amorphous calcium carbonate transformation into calcite, large calcite crystal units and rapid growth phase). The study gave new insight on proteins playing crucial role in the biomineralization of the shell.

2015-06-09 | PXD001450 | Pride

“PALAEOSHELLOMICS” REVEALS THE USE OF FRESHWATER MOTHER-OF-PEARL IN PREHISTORIC ORNAMENTS.

Project description:Palaeoproteomics analysis of mollusc shells (Modiolus modiolus, Ostrea edulis, Unio pictorum, Margaritifera margaritifera, Unio crassus, Pseudunio auricularius) and shell ornaments (double-buttons) from Neolithic European sites (Havnø, Hornstaad-Hörnle IA, Peştera Ungurească).

2019-05-08 | PXD011985 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data