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ITRAQ-based phospho-proteomics identifies a critical role of ATF2 in pseudorabies virus replication

ABSTRACT: Pseudorabies virus (PRV), the etiological agent of Aujeszky’s disease, is a swine pathogen of the alphaherpesvirinae subfamily resulting in devastating neurological and respiratory disorders in piglets, or abortion in pregnant sows.Here, we performed isobaric tags for relative and absolute quantitation (iTRAQ) quantitative phosphoproteomics on PRV-infected PK-15 cells, which identified 5723 phospho-peptides, corresponding to 2180 proteins, including 810 proteins showing significant changes in phosphorylation level during early PRV infection.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: Zheng-Fei Liu

LAB HEAD: Zheng-Fei Liu

PROVIDER: PXD014685 | Pride | 2022-10-13

REPOSITORIES: Pride

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Phospho-proteomics identifies a critical role of ATF2 in pseudorabies virus replication.

Jiang Fang-Fang FF Wang Ren-Qi RQ Guo Chao-Yue CY Zheng Ke K Liu Hai-Long HL Su Le L Xie Sheng-Song SS Chen Huan-Chun HC Liu Zheng-Fei ZF

Virologica Sinica 20220607 4

Pseudorabies virus (PRV), an etiological agent of pseudorabies in livestock, has negatively affected the porcine industry all over the world. Epithelial cells are reported as the first site of PRV infection. However, the role of host proteins and its related signaling pathways in PRV replication is largely unclear. In this study, we performed a quantitative phosphoproteomics screening on PRV-infected porcine kidney (PK-15) epithelial cells. Totally 5723 phosphopeptides, corresponding to 2180 pro ...[more]

PMID: 35688418

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