Proteomics

Dataset Information

0

Microflow SWATH data analysed with DIA-NN


ABSTRACT: We have developed DIA-NN, an integrated software suite for fully-automated processing of raw SWATH/DIA data. DIA-NN enables deep and confident proteome coverage with fast chromatographic methods, paving the way for a new generation of high-throughput proteomics. This data set illustrates the capabilities of DIA-NN when analysing microflow SWATH data acquired with 19 - 23 minute gradients as well as the use of DIA-NN to generate spectral libraries directly from SWATH/DIA data.

INSTRUMENT(S): TripleTOF 6600

ORGANISM(S): Homo Sapiens (human) Saccharomyces Cerevisiae (baker's Yeast)

TISSUE(S): Blood Plasma, Cell Culture

SUBMITTER: Vadim Demichev  

LAB HEAD: Markus Ralser

PROVIDER: PXD014690 | Pride | 2019-11-06

REPOSITORIES: Pride

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Publications

DIA-NN: neural networks and interference correction enable deep proteome coverage in high throughput.

Demichev Vadim V   Messner Christoph B CB   Vernardis Spyros I SI   Lilley Kathryn S KS   Ralser Markus M  

Nature methods 20191125 1


We present an easy-to-use integrated software suite, DIA-NN, that exploits deep neural networks and new quantification and signal correction strategies for the processing of data-independent acquisition (DIA) proteomics experiments. DIA-NN improves the identification and quantification performance in conventional DIA proteomic applications, and is particularly beneficial for high-throughput applications, as it is fast and enables deep and confident proteome coverage when used in combination with  ...[more]

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