Lysosomal proteomics by affinity purification-mass spectrometry

ABSTRACT: The aims of this project is to establish a comprehensive proteomic map of the lysosomes using isolated lysosomes via affinity purification. Affinity-purified mitochondria was used as a control to demonstrate the organelle specificity. In this study, we identified several lipid transporters that are associated with the lysosome and further characterized their roles in sterol-dependent mTORC1 regulation.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell

SUBMITTER: Chun-Yan Lim  

LAB HEAD: Roberto Zoncu

PROVIDER: PXD014733 | Pride | 2019-09-27

REPOSITORIES: Pride