BS3 crosslinking of GRC3 and Las1

ABSTRACT: Ribosome assembly is a complex process involving the coordination of multiple enzymes. A recently discovered endoribonuclease (RNase), Las1, and the poly-nucleotide kinase (PNK), Grc3, assemble into a complex. To attempt to understand the coordination of the two enzymes in this complex, we performed chemical crosslinking and mass spectrometry and also solved a series of cryo-electron microscopy structures of RNase PNK in multiple conformational states.

INSTRUMENT(S):

ORGANISM(S): Chaetomium Thermophilum

SUBMITTER: Jason Williams  

LAB HEAD: Jason G. Williams

PROVIDER: PXD014738 | Pride | 2019-09-11

REPOSITORIES: Pride