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Quantitative interactome analysis of PHD2

ABSTRACT: To identify novel regulator of EGLN1/PHD2, we performed label-free quantitative interactome analysis. Stable Hela cell lines expressing Flag-tagged PHD2 and control vector were generated. Immunoprecipitation and mass spectrometry analysis were performed to identify novel interactors of PHD2 followed by validation and functional analysis.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Yue Chen

LAB HEAD: Yue Chen

PROVIDER: PXD014757 | Pride | 2021-09-08

REPOSITORIES: Pride

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Label-Free Interactome Analysis Revealed an Essential Role of CUL3-KEAP1 Complex in Mediating the Ubiquitination and Degradation of PHD2.

Luo Ang A Chen Yue Y

Journal of proteome research 20191206 1

Prolyl hydroxylase domain-containing protein 2 (PHD2/EGLN1) is a key regulatory enzyme that plays a fundamental role in the cellular hypoxic response pathway, mediating proline hydroxylation-dependent protein degradation of selected target proteins. However, the regulation of PHD2 homeostasis at the protein level is not well understood. Here, we perform label-free quantitative interactome analysis through immunoprecipitation coupled with mass spectrometry analysis. To minimize the side effects c ...[more]

PMID: 31763849

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