Dataset Information

Peptidomics of the Asian Citrus Psyllid

ABSTRACT: The aim of this study was to develop methods for the extraction of endogenous peptides from the Asian citrus psyllid, vector of the bacteria that cause the major citrus disease Huanglongbing. In addition, the native peptidome of the Asian citrus psyllid is described, with an emphasis on candidate neuropeptides.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Diaphorina Citri (asian Citrus Psyllid)

TISSUE(S): Whole Body

SUBMITTER: Laura Fleites

LAB HEAD: Michelle L. Heck

PROVIDER: PXD014801 | Pride | 2020-03-19

REPOSITORIES: Pride

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Publications

Peptidomics Approaches for the Identification of Bioactive Molecules from Diaphorina citri.

Fleites Laura A LA Johnson Richard R Kruse Angela R AR Nachman Ronald J RJ Hall David G DG MacCoss Michael M Heck Michelle L ML

Journal of proteome research 20200302 4

Huanglongbing (HLB), a deadly citrus disease, is primarily associated with Candidatus Liberibacter asiaticus (CLas) and spread by the hemipteran insect Diaphorina citri. Control strategies to combat HLB are urgently needed. In this work, we developed and compared workflows for the extraction of the D. citri peptidome, a dynamic set of polypeptides produced by proteolysis and other cellular processes. High-resolution mass spectrometry revealed bias among methods reflecting th ...[more]

PMID: 32037832

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Project description:Citrus greening disease, also known as Huanglongbing (HLB), is the most serious disease of citrus plants. It is associated with the Gram-negative bacterium ‘Candidatus Liberibacter asiaticus’ (CLas), which is transmitted between host plants by the hemipteran insect vector Diaphorina citri in a circulative, propagative manner involving specific interactions with various insect tissues, including the hemolymph, fluid that occupies the body cavity akin to insect blood. High resolution quantitative mass spectrometry was performed to investigate the effect of CLas exposure on D. citri hemolymph at the proteome level. In contrast to the broad proteome effects on hundreds of proteins and a diverse array of metabolic pathways previously reported in gut and whole insect proteome analyses, the effect of CLas on the hemolymph was observed to be highly specific, restricted to key immunity and metabolism pathways, and lower in magnitude than that previously observed in the whole insect body and gut. Vitellogenins were abundantly expressed and CLas-responsive. Gene-specific RNA expression analysis suggests that these proteins are expressed in both male and female insects, and may have roles outside of reproductive vitellogenesis. Proteins for fatty acid synthesis were found to be up-regulated, along with metabolic proteins associated with energy production, supported at the organismal level by the previously published observation that D. citri individuals experience a higher level of hunger when reared on CLas-infected plants. Prediction of post-translational modifications identified hemolymph proteins with phosphorylation and acetylation upon CLas exposure. Proteins derived from the three most prominent bacterial endosymbionts of the psyllid were also detected in the hemolymph, and several of these have predicted secretion signals. A DNAK protein, the bacterial HSP70, detected in the hemolymph expressed from Wolbachia pipientis was predicted to encode a eukaryotic nuclear localization signal. Taken together, these data show specific changes to immunity and metabolism in D. citri hemolymph involving host and endosymbiont proteins. These data provide a novel context for proteomic changes seen in other D. citri tissues in response to CLas and align with organismal data on the effects of CLas on D. citri metabolism and reproduction.

Dataset Information

Peptidomics of the Asian Citrus Psyllid

Publications

Peptidomics Approaches for the Identification of Bioactive Molecules from <i>Diaphorina citri</i>.

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