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Proteomic profiling of PTMs at HP1 protein

ABSTRACT: The levels of phosphorylated HP1beta peptide forms in HeLa cells after irradiation and SAHA treatment were examined.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hela Cell

SUBMITTER: Gabriela Lochmanova

LAB HEAD: Zbynek Zdrahal

PROVIDER: PXD014802 | Pride | 2019-09-25

REPOSITORIES: Pride

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Publications

DNA Damage Changes Distribution Pattern and Levels of HP1 Protein Isoforms in the Nucleolus and Increases Phosphorylation of HP1β-Ser88.

Legartová Soňa S Lochmanová Gabriela G Zdráhal Zbyněk Z Kozubek Stanislav S Šponer Jiří J Krepl Miroslav M Pokorná Pavlína P Bártová Eva E

Cells 20190917 9

The family of heterochromatin protein 1 (HP1) isoforms is essential for chromatin packaging, regulation of gene expression, and repair of damaged DNA. Here we document that γ-radiation reduced the number of HP1α-positive foci, but not HP1β and HP1γ foci, located in the vicinity of the fibrillarin-positive region of the nucleolus. The additional analysis confirmed that γ-radiation has the ability to significantly decrease the level of HP1α in rDNA promoter and rDNA encoding 28S rRNA. By mass spec ...[more]

PMID: 31533340

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