Proteomics

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Thermococcus kodakarensis proteins expressed at various temperatures reveals changes in RNA modifications

ABSTRACT: Thermococcus kodakarensis is a thermophilic archaea used in this study to determine how changes in growth temperature affect protein expression. This organism was chosen, in part, due to the simplified proteome and potential protein stability gained when analyzing proteins at room temperature. Two temperatures were used as well as one knockout cell line to determine the differences in protein expression. Specifically, changes in protein expression levels were used in conjunction with other data to determine the changing levels of RNA modification based on expression temperature.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Thermococcus Kodakarensis Kod1

TISSUE(S): Cell Culture

SUBMITTER: Michaella Levy  

LAB HEAD: Laurence Florens

PROVIDER: PXD014814 | Pride | 2020-02-17

REPOSITORIES: Pride

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N<sup>4</sup>-acetylcytidine (ac<sup>4</sup>C) is an ancient and highly conserved RNA modification that is present on tRNA and rRNA and has recently been investigated in eukaryotic mRNA<sup>1-3</sup>. However, the distribution, dynamics and functions of cytidine acetylation have yet to be fully elucidated. Here we report ac<sup>4</sup>C-seq, a chemical genomic method for the transcriptome-wide quantitative mapping of ac<sup>4</sup>C at single-nucleotide resolution. In human and yeast mRNAs, ac<s  ...[more]

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