Identification of cellular CDK9 substrates using an analog-sensitive cell line

ABSTRACT: Analog-sensitive kinase technology allows for specific inhibition of a given kinase. Here we utilize an analog-sensitive CDK9 cell line to determine the impact of CDK9 inhibition on the phospho-proteome of human cells. Specifically, we aim to identify substrates of CDK9 in a quantitative way using stable isotope labeling by amino acids in cell culture (SILAC) followed by LC-MS/MS.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): B Cell

SUBMITTER: Ignasi Forne  

LAB HEAD: Prof. Dr. Dirk Eick

PROVIDER: PXD014825 | Pride | 2019-11-11

REPOSITORIES: Pride