DETECTION OF ODF1 IN KIDNEY MEDULLA

ABSTRACT: ODF1 has been described as an exclusively expressed testicular protein and is located in the outer dense fibers along the sperm tail. ODF1 has been involved in the sperm motility and in the development of the flagellum, but the function of ODF1 is not already clear. Applying western blot, immunofluorescence, and RT-PCR techniques we identified the protein and mRNA of ODF1 in the rat kidney. Finally, the presence of ODF1 in kidney has been confirmed through proteomic analysis using mass spectrometry. The results derived from these different complementary approaches indicate that, to our knowledge and for the first time, ODF1 is demonstrated to be present in an additional organ different to testis.

INSTRUMENT(S):

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Testis, Kidney

SUBMITTER: María Eugenia Cabrillana  

LAB HEAD: Miguel Walter Fornés

PROVIDER: PXD014848 | Pride | 2020-05-27

REPOSITORIES: pride