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Phosphoproteomic analysis of neonatal regenerative myocardium revealed important roles of CHK1 via activating mTORC1/P70S6K pathway

ABSTRACT: Restoring the regenerative capacity of myocardium, which presents shortly in the newborns but lost in adulthood, is one of the therapeutic options for myocardial repair post various insults. In this work, we performed the phosphoproteomic analysis on neonatal regenerative myocardium and explored the Kinase-substrate network in neonatal myocardium to define potential signaling, which might be responsible for the transient regenerative capacity in the newborn mice.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Heart

SUBMITTER: Yiwei Cheng

LAB HEAD: Xuejiang Guo

PROVIDER: PXD014865 | Pride | 2020-02-28

REPOSITORIES: Pride

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Phosphoproteomic Analysis of Neonatal Regenerative Myocardium Revealed Important Roles of Checkpoint Kinase 1 via Activating Mammalian Target of Rapamycin C1/Ribosomal Protein S6 Kinase b-1 Pathway.

Fan Yi Y Cheng Yiwei Y Li Yafei Y Chen Bingrui B Wang Zimu Z Wei Tianwen T Zhang Hao H Guo Yueshuai Y Wang Qiming Q Wei Yongyue Y Chen Feng F Sha Jiahao J Guo Xuejiang X Wang Liansheng L

Circulation 20200226 19

<h4>Background</h4>In mammals, regenerative therapy after myocardial infarction is hampered by the limited regenerative capacity of adult heart, whereas a transient regenerative capacity is maintained in the neonatal heart. Systemic phosphorylation signaling analysis on ischemic neonatal myocardium might be helpful to identify key pathways involved in heart regeneration. Our aim was to define the kinase-substrate network in ischemic neonatal myocardium and to identify key pathways involved in he ...[more]

PMID: 32098494

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