Proteomics

Dataset Information

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Plasmodium falciparum exported proteins co-immunoprecipitation LC-MS/MS

ABSTRACT: The malaria parasite, Plasmodium falciparum, traffics the virulence protein P. falciparum erythrocyte membrane protein 1 (PfEMP1) to the surface of infected red blood cells (RBCs) via membranous organelles known as the Maurer’s clefts. We developed a method for efficient enrichment of Maurer’s clefts and profiled the protein composition of this trafficking organelle. We identified 18 previously uncharacterised or poorly characterised Maurer’s cleft proteins. Transfectants expressing GFP-fusions of 7 of these proteins and confirmed their Maurer’s cleft location. Co-immunoprecipitation and mass spectrometry identified interacting partners of these Maurer’s clefts proteins, providing a network map of protein-protein associations. We identified two key clusters of proteins that may function in the loading and unloading of PfEMP1 into and out of the Maurer’s clefts.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Plasmodium Falciparum (isolate 3d7)

TISSUE(S): Blood

SUBMITTER: Emma McHugh  

LAB HEAD: Matthew WA Dixon

PROVIDER: PXD014873 | Pride | 2020-02-20

REPOSITORIES: Pride

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Publications

Role of Plasmodium falciparum Protein GEXP07 in Maurer's Cleft Morphology, Knob Architecture, and P. falciparum EMP1 Trafficking.

McHugh Emma E   Carmo Olivia M S OMS   Blanch Adam A   Looker Oliver O   Liu Boyin B   Tiash Snigdha S   Andrew Dean D   Batinovic Steven S   Low Andy J Y AJY   Cho Hyun-Jung HJ   McMillan Paul P   Tilley Leann L   Dixon Matthew W A MWA  

mBio 20200317 2

The malaria parasite <i>Plasmodium falciparum</i> traffics the virulence protein <i>P. falciparum</i> erythrocyte membrane protein 1 (<i>Pf</i>EMP1) to the surface of infected red blood cells (RBCs) via membranous organelles, known as the Maurer's clefts. We developed a method for efficient enrichment of Maurer's clefts and profiled the protein composition of this trafficking organelle. We identified 13 previously uncharacterized or poorly characterized Maurer's cleft proteins. We generated tran  ...[more]

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