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Characterization of the CORVET complexes of Tetrahymena thermophila

ABSTRACT: In endolysosomal networks, two hetero-hexameric tethers called HOPS and CORVET are found widely throughout eukaryotes. The ciliate Tetrahymena thermophila is a unicellular organism with elaborate endolysosomal pathways. Curiously, Tetrahymena and related protozoa have lost HOPS. Tetrahymena encodes multiple paralogs of most CORVET subunits, assembled into six distinct hexameric complexes, including a distinct Vps8 subunit. Here we analyze the composition of these complexes immunoisolated by affinity capture using the Vps8-FLAG paralogs as bait.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Tetrahymena Thermophila Cu428

TISSUE(S): Permanent Cell Line Cell

DISEASE(S): Disease Free

SUBMITTER: Martin Zoltner

LAB HEAD: Martin Zoltner

PROVIDER: PXD014895 | Pride | 2019-12-19

REPOSITORIES: Pride

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